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Ling YANG, Rui ZHU, Qingjing ZHU, Dan DAN, Jin YE, Keshu XU, Xiaohua HOU
Frontiers of Medicine 2009, Volume 3, Issue 1, Pages 36-40 doi: 10.1007/s11684-009-0020-y
Keywords: liver cirrhosis beta-elemene hepatic stellate cells angiotensin II receptor angiotensin type 1
Ruoxi Zhang, Jing Chen, Diangang Liu, Yu Wang
Frontiers of Medicine 2019, Volume 13, Issue 3, Pages 398-408 doi: 10.1007/s11684-019-0689-5
Keywords: portal hypertension cirrhosis urotensin II palosuran hepatic stellate cell
Immunohistochemical characterization of hepatic stem cell-related cells in developing human liver
XU Jun, HU Yong, WANG Jian, ZHANG Taiping, ZHOU Ji, YU Hongyu
Frontiers of Medicine 2007, Volume 1, Issue 3, Pages 264-268 doi: 10.1007/s11684-007-0050-2
Keywords: origin Little mesenchymal tissue cords/mesenchymal tissue CD34
Metformin and metabolic diseases: a focus on hepatic aspects
Juan Zheng,Shih-Lung Woo,Xiang Hu,Rachel Botchlett,Lulu Chen,Yuqing Huo,Chaodong Wu
Frontiers of Medicine 2015, Volume 9, Issue 2, Pages 173-186 doi: 10.1007/s11684-015-0384-0
Keywords: metformin diabetes hepatic steatosis inflammatory response insulin resistance
Effect of traditional Chinese medicine combined with Western therapy on primary hepatic carcinoma: a
Li Ma, Bin Wang, Yuanxiong Long, Hanmin Li
Frontiers of Medicine 2017, Volume 11, Issue 2, Pages 191-202 doi: 10.1007/s11684-017-0512-0
Primary hepatic carcinoma (PHC) is a common malignant tumor in China.review with meta-analysis aimed to assess the effect of TCM combined with Western therapy on primary hepatic
Keywords: traditional Chinese medicine primary hepatic carcinoma meta-analysis
Wu Limao,Li Lianda,Liu Hong,Ning Keyong,Li Yikui
Strategic Study of CAE 2004, Volume 6, Issue 7, Pages 34-42
Keywords: bone marrow stem cells autologous transplantation liver injury liver fibrosis guiyuan fang
Beneficial effects of preventive cholecystectomy in patients with hepatic cancer
LIU Anzhong, LI Jun, HUA Huwei
Frontiers of Medicine 2008, Volume 2, Issue 2, Pages 139-142 doi: 10.1007/s11684-008-0026-x
Paeoniflorin prevents hepatic fibrosis of by inhibiting TGF-β1 production from macrophages in mice
CHU Deyong, LI Conglei, SHEN Jilong, WU Qiang
Frontiers of Medicine 2008, Volume 2, Issue 2, Pages 154-165 doi: 10.1007/s11684-008-0029-7
Keywords: cercariae collagen hyaluronic OPMCM soluble
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen
Frontiers of Medicine 2015, Volume 9, Issue 1, Pages 90-99 doi: 10.1007/s11684-015-0390-2
Gene therapy provides a potential cure for hemophilia B, and significant progress has been achieved in liver-directed gene transfer mediated by adeno-associated viral vectors. Recent clinical trials involving the use of a self-complementary adeno-associated virus serotype 8-human codon-optimized factor IX (AAV8-hFIXco) vector demonstrated encouraging efficacy with hFIX expression stabilized at 1% to 6% of normal level in patients, but safety concerns related to high vector doses are still present. Thus, further improvement of AAV vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B gene therapy. In this study, to obtain a higher expression level of hFIX that potentiates the coagulant capacity of recipients, human FIX expression vector was optimized by upgrading the codon adaption index and adjusting the GC content, inserting a Kozak sequence (GCCACC), and introducing a gain-of-function mutation, R338L (FIX Padua). The efficiency of the published and the presently constructed cassettes was compared through in vivo screening. In addition, the regulatory elements that control the FIX gene expression in these cassettes were screened for liver-specific effectiveness. Among all the constructed cassettes, scAAV-Pre-hFIXco-SIH-R338L, which was the construct under the control of the prothrombin enhancer and prealbumin promoter, resulted in the highest level of coagulant activity, and the expression levels of two constructed cassettes (scAAV-Chi-hFIXco-SIH-R338L and scAAV-Pre-hFIXco-SIH-R338L) were also higher than that of the published cassette (scAAV-LP1-hFIXco-SJ). In summary, our strategies led to a substantial increase in hFIX expression at the protein level or a remarkably elevated coagulant activity. Thus, these reconstructs of hFIX with AAV vector may potentially contribute to the creation of an efficacious gene therapy of hemophilia B.
Keywords: factor IX hemophilia B liver-specific regulatory elements hydrodynamic gene transfer
Expression of integrin in hepatic fibrosis and intervention of resveratrol
Jianye WU, Chuanyong GUO, Jun LIU, Xuanfu XUAN
Frontiers of Medicine 2009, Volume 3, Issue 1, Pages 100-107 doi: 10.1007/s11684-009-0013-x
Keywords: liver fibrosis integrin-β1 resveratrol tumor growth factor-β tissue inhibitor of metalloproteinase-1
Zhi-Yong ZHANG, Xiao-Ping CHEN, Qi-Ping LU
Frontiers of Medicine 2010, Volume 4, Issue 3, Pages 317-322 doi: 10.1007/s11684-010-0035-4
Keywords: hepatic ischemia-reperfusion digestive tract congestion cholecystokinin vasoactive intestinal peptide
Eric C.H. Lai
Frontiers of Medicine 2013, Volume 7, Issue 4, Pages 520-522 doi: 10.1007/s11684-013-0302-2
Yanli LIU, Rong LIU, Cheng ZHEN, Quanfang GUO, Liping WU, Zhaoxi DING, Yushun BI, Zhiyu LIU
Frontiers of Medicine 2009, Volume 3, Issue 1, Pages 91-95 doi: 10.1007/s11684-009-0011-z
Keywords: Fuganling granula immunological liver injury bacille calmette guerin lipopolysaccharide mice
Glucagon-like peptide-2 exhibits protective effect on hepatic ischemia-reperfusion injury in rats
Naci Topaloğlu,Adem Küçük,Şule Yıldırım,Mustafa Tekin,Havva Erdem,Mustafa Deniz
Frontiers of Medicine 2015, Volume 9, Issue 3, Pages 368-373 doi: 10.1007/s11684-015-0403-1
Keywords: ischemia/reperfusion liver glucagon-like peptide-2 alanine aminotransferase
Jianguo LIU, Zhe ZHANG, Jiechang GAO, Jiwen XIE, Lin YANG, Shenjun HU
Frontiers of Medicine 2011, Volume 5, Issue 1, Pages 101-105 doi: 10.1007/s11684-011-0111-4
Keywords: liver fibrosis beta-elemene endotoxin CD14
Title Author Date Type Operation
Influence of β-elemene on the secretion of angiotensin II and expression of AT1R in hepatic stellatecells
Ling YANG, Rui ZHU, Qingjing ZHU, Dan DAN, Jin YE, Keshu XU, Xiaohua HOU
Journal Article
Urotensin II receptor antagonist reduces hepatic resistance and portal pressure through enhanced eNOS-dependent
Ruoxi Zhang, Jing Chen, Diangang Liu, Yu Wang
Journal Article
Immunohistochemical characterization of hepatic stem cell-related cells in developing human liver
XU Jun, HU Yong, WANG Jian, ZHANG Taiping, ZHOU Ji, YU Hongyu
Journal Article
Metformin and metabolic diseases: a focus on hepatic aspects
Juan Zheng,Shih-Lung Woo,Xiang Hu,Rachel Botchlett,Lulu Chen,Yuqing Huo,Chaodong Wu
Journal Article
Effect of traditional Chinese medicine combined with Western therapy on primary hepatic carcinoma: a
Li Ma, Bin Wang, Yuanxiong Long, Hanmin Li
Journal Article
Studies of the Guiyuan Fang and Autologous Bone Marrow Stem Cells Transplantation on Hepatic Injury and
Wu Limao,Li Lianda,Liu Hong,Ning Keyong,Li Yikui
Journal Article
Beneficial effects of preventive cholecystectomy in patients with hepatic cancer
LIU Anzhong, LI Jun, HUA Huwei
Journal Article
Paeoniflorin prevents hepatic fibrosis of by inhibiting TGF-β1 production from macrophages in mice
CHU Deyong, LI Conglei, SHEN Jilong, WU Qiang
Journal Article
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen
Journal Article
Expression of integrin in hepatic fibrosis and intervention of resveratrol
Jianye WU, Chuanyong GUO, Jun LIU, Xuanfu XUAN
Journal Article
Effect of salvia miltiorrhiza pretreatment on the CCK and VIP expression in hepatic ischemia-reperfusion-induced
Zhi-Yong ZHANG, Xiao-Ping CHEN, Qi-Ping LU
Journal Article
laparoscopic hepatectomy: comments on “Expert Consensus on Laparoscopic Hepatectomy (2013 Version) by National Hepatic
Eric C.H. Lai
Journal Article
Experimental study on the efficacy of Fuganling granula on protecting against immunological hepatic injury
Yanli LIU, Rong LIU, Cheng ZHEN, Quanfang GUO, Liping WU, Zhaoxi DING, Yushun BI, Zhiyu LIU
Journal Article
Glucagon-like peptide-2 exhibits protective effect on hepatic ischemia-reperfusion injury in rats
Naci Topaloğlu,Adem Küçük,Şule Yıldırım,Mustafa Tekin,Havva Erdem,Mustafa Deniz
Journal Article